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"This manual complements Simpson’s Proteins and Proteomics manual, with a comprehensive collection of methods for protein purification from a variety of source preparations. The chapters include detailed protocols, methods for optimizing the performance of experiments, discussion of potential pitfalls, and troubleshooting advice."
The Sequencing of genomes has opened the door for exploration of the proteome, the many thousands of proteins and their interactions in a given organism. Reliable, bench-tested protocols are required for identifying, isolating, and quantifying proteins in a given cell or pathway and for performing functional assays. Proteins and Proteomics: A Laboratory Manual by Richard Simpson, a leading authority in the field, provides the first authoritative, wide-ranging, ...