Genome Analysis in Farm Animals

Contents of the Book PART-I DNA MARKERS FOR GENOME ANALYSIS OF FARM ANIMALS 1. Role of DNA Markers in Genome Analysis of Farm Animals 2. Restriction Fragment Length Polymorphism (RFLP) Analysis 3. Origin, Evolution, Distribution and Application of microsatellite markers 4. Single Nucleotide Polymorphisms: Detection Methodologies 5. Methods in Gene Mapping 6. Methods of Differential Gene Expression Analysis PART-II TOOLS FOR GENOME ANALYSIS IN FARM ANIMALS: PRACTICAL ASPECTS 7. Preparation of Metaphase Chromosomes and Banding Techniques 8. FISH as a Diagnostic Tool in Chromosome and Genome Analysis 9. Extraction and Quantification of Nucleic Acids 10. Polymerase Chain Reaction: Basic Principles 11. Poly erase Chain Reaction: Practical Aspects 12. Agarose Gel Electrophoresis 13. Denaturing Polyacrylamide Gel Electrophoresis 14. Non-denaturing Page: Single Strand Conformation Polymorphism (SSCP) 15. DNA Sequencing 16. Real time PCR and its Applications PART-III BIOINFORMATIC TOOLS FOR GENOME ANALYSIS IN FARM ANIMALS 17. Designing Oligonucleotide Primers for Genome Analysis 18. DNA Sequence Analysis 19. Molecular Genetic and Quantitative Data Analysis