Plant Genetic Transformation and Molecular Markers

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Large number of transgenic crop plants have now been released for commercial production using transformation technologies. Virus-mediated, chemical mediated microinjection, electroporation, particle-bombardment and other transformation methods are routinely used. However, problems are associated with stable integration and reliable expression of the DNA after integration. Genetic analysis of plants is a basic requirement for studies in genetics and plant breeding. Marker, be it morphological, biochemical or molecular, play a key role in genetic studies. With the increasing availability of information on genome sequences for different plant species, the sequence specific molecular markers (e.g. SNP, TRAP) are becoming more important and useful over the random DNA Markers (e.g. RAPD, DAF). This book provides deep insight into the molecular techniques of genetic transformation and the application of molecular markers, used to scrutinize DNA Sequence variation(s) in and among the crop species and create new sources of genetic variation by introducing new and favourable traits from landraces and related crop species. Examples are from crop and horticultural plants including rice, sugarcane etc. The book also covers nanotechnology and its application in plant biotechnology.

This book is unique in several respects, the most important being the attempt to deal with all the topics in depth and to find molecular aspects of different processes of plant genetic transformation and molecular markers and is aimed towards the students, researchers and teachers in the field of plant biotechnology in universities, agricultural and horticultural, engineering institutions and industries.

ABOUT THE AUTHOR Ashwani Kumar

Ashwani Kumar, a Congress Party member of Rajya Sabha from Punjab, and Chairman, AICC Vichar Vibhag, is a practicing Senior Advocate at the Supreme Court and was appointed Additional Solicitor General of India in 1990 at the age of 37. He has appeared before the Court in landmark cases which include the Bhopal Gas case, the Gujarat Presidential Reference case and the recently decided Electoral Reforms case. At 33, he first represented India as a non-official member of the Indian Delegation to the UN General Assembly in1986 and later again in 1996. Ashwani Kumar ahs also served as a Spokesperson of the Congress Party and as member of the Congress Party's Economic Policy Introspection Group. He has travelled extensively and was as Visiting Fellow at the Centre for Strategic and International Studies (CSIS) in Washington. He has also lectured at the University of Nice. He is currently a member of the Standing Committee of Parliament on Commerce and has also served as a member of the Standing Committee of Parliament on Energy. Ashwani Kumar is the son of Late Prabodh Chandra, a veteran freedom fighter, distinguished parliamentarian and Congress leader from Punjab.