Practical Plant Physiology

Book contain great number of technically feasible, well designed experiments covering nearly all the areas of basic and modern plant physiology. Each experiment is preceded by a brief theoretical background, principle, and simplified protocol. Each experiment is given with well defined purpose and uses of the experiment in agricultural Research. Each reagent in an experiment is given with purpose associated with principle.

There are seven tests in the book contain about 420 questions from the experiments with the answers at the last. Each test questions contains fill ups, choose the best answer, match it and true/False from the practical of plant physiology with interesting manner. This book is essential for all the students of science faculties in agriculture to understand the physiology practical in an easy manner. This practical guide is highly useful for the students to encourage the interest of functions of plants at organ, cellular and molecular level. Practical plant physiology (PPP) is going to be one of the best practical/laboratory guide for all the research scholars in agriculture.

Contents: Preface. I. Plant water relations: 1. Preparation of solutions. 2. Estimation of water potential by Tissue weight method. 3. Estimation of water potential by Chardakov’s method or falling drop method. 4. Estimation of water potential by pressure Chamber apparatus. 5. Calculation of Osmotic potential and Osmotic adjustment. 6. Demonstration of plasmolysis. 7. Calculation of stomatal index and Frequency. 8. Measurement of leaf temperature, diffusive resistance and transpiration by steady state porometer. II. Productive physiology: 9. Measurement of gas exchange parameters-PPS. 10. Estimation of leaf area-different methods. 11. Estimation of photosynthetic pigments. 12. Estimation of chlorophyll Index by SPAD Meter-Minolta SPAD 502. 13. Estimation of chlorophyll content without homogenization. 14. Estimation of lycopene. 15. Assessment of light use efficiency. 16. Estimation of soluble protein. 17. Estimation of nitrate reductase (NR) enzyme activity. 18. Estimation of nitrite reductase (NIR) Enzyme activity. 19. Estimation of IAA Oxidase (IAAO) activity. 20. Estimation of Starch. 21. Estimation of total Sugars. 22. Estimation of reducing Sugars. 23. Estimation of Titrable Acidity. 24. Estimation of TSS by Refractometer. 25. Estimation of ascorbic acid content. 26. Assessment of photosynthetic efficiency of crop plants. III. Mineral Nutrition: 27. Diagnosis of nutritional disorders in crop plants. 28. Physiological disorders in crop plants. 29. Diagnosis of nutrient deficiencies through rapid tissue test. 30. Estimation of nitrogen-kjeldhal method. 31. Estimation of phosphorus. 32. Atomic absorption spectrometry. IV. Stress physiology: 33. Induction of drought by different methods. 34. Estimation of relative water content. 35. Estimation of chlorophyll stability index. 36. Estimation of proline. 37. Estimation of peroxidase activity. 38. Estimation of catalase activity. 39. Estimation of super Oxide dismutase (SOD) activity. 40. Estimation of poly phenol oxidase (PPO) activity. 41. Estimation of Amylase activity. 42. Estimation of polygalacturaonase activity. 43. Estimation of pectin Methl Esterase activity. 44. Estimation of Phenylalanine Ammonia Lyase activity. 45. Estimation of sucrose phosphate synthase (SPS) activity. 46. Estimation of invertase activity. 47. Estimation of lipid peroxidation (MDA content). 48. Estimation of total phenolics. 49. Quantification of epicuticular wax (ECW). 50. Determination of membrane stability index-EC method. 51. Determination of membrane integrity by spectrophotometer method. 52. Sullivan’s heat tolerance test. V. Hormone physiology: 53. Demonstration of crop response to growth regulators. 54. Bioassay of auxin. 55. Bio-assay for gibberellins. 56. Bio-assay for cytokinin. 57. Bio-assay for ethylene. 58. Growth analysis. VI. Molecular physiology: 59. Molecular physiology isolation of plant genomic DNA. 60. Agarose gel electrophoresis of DNA. 61. Isolation of plasmid DNA from E. Coli. 62. Analysis of proteins by SDS-page. 63. Amplification of DNA by polymerase chain reaction. References. Appendices.