This is a concise laboratory hand book for preparation, micro-analytical estimation and characterization of biological material. It is one of the few books that give practical laboratory methodology. The methods are detailed completely in a step by step format that allows the laboratory workers to perform any procedure without reference to secondary sources. Not only are all reagents specified, but the exact methods of preparation are delineated so that no further calculations are necessary for the biochemist, micro biologist, immunologist, pharmacology. The main topics, dealt in this volume are: Cryostats; Decalcification; Injection Techniques; Tissues Requiring Special Treatment or Techniques; Stains for Cytoplasmic Constituents; Proteins and Amino Acids; Nucleoproteins (DNA & RNA); Enzymes; Carbohydrates; Identification of Lipids; How to Study Protozoans; Ecological Techniques; DNA Preparation; Protein Isolation; Isolation of Free Fatty Acids; Quantitative Micro-precipitation; Quick Methods of Identification.
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